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KMID : 0364820200560030318
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Korean Journal of Microbiology
2020 Volume.56 No. 3 p.318 ~ p.320
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Complete genome sequence of Peanut mottle virus identified from soybean (Glycine max L.) using RNA-sequencing
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Jo Yeon-Hwa
Choi Ho-Seong
Choi Soo-Yeon
Kim Sang-Min
Lee Bong-Choon
Cho Won-Kyong
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Abstract
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To identify viruses infecting soybean (Glycine max L.), we collected soybean leaf samples showing viral disease symptoms and subjected them to RNA-sequencing. De novo transcriptome assembly followed by BLAST search identified Peanut mottle virus (PeMoV) belonging to the genus Potyvirus in the family Potyviridae. We obtained the complete genome of PeMoV isolate Daegu with 9,708 nucleotides encoding polypeptide (3,099 aa) and pretty interesting potyviridae ORF (PIPO) (74 aa) proteins. The phylogenetic analysis using 10 available complete genomes for PeMoV isolates demonstrated that PeMoV isolate Daegu is grouped with five other PeMoV isolates from Korea, China, Kenya, and Brazil.
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KEYWORD
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genome, peanut mottle virus, RNA-seq, soybean, virus
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